 CellGraph, clock-gene Tool |
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Introduce
. Live Cell Bioluminescent Imaging System
. cell 1개의 미약한 발광을 검출하기 위해 개발된 Imager
. 집광효율이 높은 광학계와 최고 level의 절대감도를 가진 CCD로
미약한 발광검출도 可
. incubator탑재 ; 장기간 배양 가능
. CO2 gas 공급 가능
. Firefly Luciferase처럼 발광단백질의 유전자를 이용한
promoter의 전사활성측정을 single cell level에서
연속적으로 측정 가능 |
CellGraph |
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Key Feathers & Benefit
- Ultra-high sensitivity
CCD Camera ; High QE (Quantum Efficiency, >90%), Low noise (-85℃),
Signal amplification by EM (Electron Multiplying)
Optical System ; Higher NA lens, Designing for minimizing of light loss
- Blocking of Outside light ; Sample stage and Optical system into Dark box
- Incubation System for Long Term Cell Culturing ; 37℃, CO2 gas
- Focus Adjustment ; Electrically operated Z-axis of stage for focus
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Stable line (Bma I1 Promoter : Luciferase/rat1)
Y. Tanahashi (Department of Physiology, Hokkaido University Graduate School of Medicine, Japan) |
2) Transient transfected cells (Bma I1 Promoter : Luciferase/NIH3T3)
Y. Nakajima (Cell Dynamics Research Group, Advanced Industrial Science and Technology, Japan) | |
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